Sequence- and regioselectivity in the montmorillonite-catalyzed synthesis of RNA.
Identifieur interne : 003198 ( Main/Exploration ); précédent : 003197; suivant : 003199Sequence- and regioselectivity in the montmorillonite-catalyzed synthesis of RNA.
Auteurs : Shin Miyakawa [États-Unis] ; James P. FerrisSource :
- Journal of the American Chemical Society [ 0002-7863 ] ; 2003.
Descripteurs français
- KwdFr :
- MESH :
- synthèse chimique : ARN, Dimères de pyrimidine.
- Bentonite, Catalyse, Dimères de pyrimidine.
English descriptors
- KwdEn :
- MESH :
- chemical , chemical synthesis : Pyrimidine Dimers, RNA.
- chemical , chemistry : Bentonite, Pyrimidine Dimers.
- Catalysis.
Abstract
The possible role of catalysis in forming a limited number of RNAs from activated monomers is investigated by examining the sequence- and regioselectivity in the montmorillonite-catalyzed formation of RNA dimers and trimers. The reactivity of A was similar to that of G, and C was comparable in reactivity to U. Yet the reactivity of the purine nucleotides differed from that of the pyrimidines. In the reaction of nucleotides (pN) with activated monomers (ImpN), the sequence- and regioselectivity was Pu(3')Py > Pu(3')Pu = Pu(2')Py > Pu(2')Pu. The 5'-pyrimidine initiated dimers formed less efficiently than the 5'-purine initiated dimers. Trimer formation was investigated by the synthesis of 8 dimers (pNpN) and measuring the yields of trimers formed in the reaction of each dimer with a mixture of equal molar amounts of four activated monomers. The reactivity of the dimers depended on the nucleotide attached to the 3'-end of the RNA and the regiochemistry of the phosphodiester bond. Rules based on these studies are proposed to predict the sequence- and regioselectivity of the RNAs formed in montmorillonite-catalyzed reactions. These rules are consistent with the structures of the 2-5-mers formed in the reaction of equimolar amounts of ImpA and ImpC. This research establishes that the montmorillonite catalyst limits the number of RNA oligomer isomers formed. The potential significance of these findings to the origins of life is discussed.
DOI: 10.1021/ja034328e
PubMed: 12837090
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002450
- to stream PubMed, to step Curation: 002450
- to stream PubMed, to step Checkpoint: 002301
- to stream Ncbi, to step Merge: 000198
- to stream Ncbi, to step Curation: 000198
- to stream Ncbi, to step Checkpoint: 000198
- to stream Main, to step Merge: 003232
- to stream Main, to step Curation: 003198
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Sequence- and regioselectivity in the montmorillonite-catalyzed synthesis of RNA.</title><author><name sortKey="Miyakawa, Shin" sort="Miyakawa, Shin" uniqKey="Miyakawa S" first="Shin" last="Miyakawa">Shin Miyakawa</name><affiliation wicri:level="2"><nlm:affiliation>Department of Chemistry and New York Center for Studies on the Origins of Life, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry and New York Center for Studies on the Origins of Life, Rensselaer Polytechnic Institute, Troy, NY 12180</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Ferris, James P" sort="Ferris, James P" uniqKey="Ferris J" first="James P" last="Ferris">James P. Ferris</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:12837090</idno><idno type="pmid">12837090</idno><idno type="doi">10.1021/ja034328e</idno><idno type="wicri:Area/PubMed/Corpus">002450</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002450</idno><idno type="wicri:Area/PubMed/Curation">002450</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002450</idno><idno type="wicri:Area/PubMed/Checkpoint">002301</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002301</idno><idno type="wicri:Area/Ncbi/Merge">000198</idno><idno type="wicri:Area/Ncbi/Curation">000198</idno><idno type="wicri:Area/Ncbi/Checkpoint">000198</idno><idno type="wicri:doubleKey">0002-7863:2003:Miyakawa S:sequence:and:regioselectivity</idno><idno type="wicri:Area/Main/Merge">003232</idno><idno type="wicri:Area/Main/Curation">003198</idno><idno type="wicri:Area/Main/Exploration">003198</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Sequence- and regioselectivity in the montmorillonite-catalyzed synthesis of RNA.</title><author><name sortKey="Miyakawa, Shin" sort="Miyakawa, Shin" uniqKey="Miyakawa S" first="Shin" last="Miyakawa">Shin Miyakawa</name><affiliation wicri:level="2"><nlm:affiliation>Department of Chemistry and New York Center for Studies on the Origins of Life, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry and New York Center for Studies on the Origins of Life, Rensselaer Polytechnic Institute, Troy, NY 12180</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Ferris, James P" sort="Ferris, James P" uniqKey="Ferris J" first="James P" last="Ferris">James P. Ferris</name></author></analytic><series><title level="j">Journal of the American Chemical Society</title><idno type="ISSN">0002-7863</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bentonite (chemistry)</term><term>Catalysis</term><term>Pyrimidine Dimers (chemical synthesis)</term><term>Pyrimidine Dimers (chemistry)</term><term>RNA (chemical synthesis)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN (synthèse chimique)</term><term>Bentonite ()</term><term>Catalyse</term><term>Dimères de pyrimidine ()</term><term>Dimères de pyrimidine (synthèse chimique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemical synthesis" xml:lang="en"><term>Pyrimidine Dimers</term><term>RNA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Bentonite</term><term>Pyrimidine Dimers</term></keywords><keywords scheme="MESH" qualifier="synthèse chimique" xml:lang="fr"><term>ARN</term><term>Dimères de pyrimidine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Catalysis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Bentonite</term><term>Catalyse</term><term>Dimères de pyrimidine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The possible role of catalysis in forming a limited number of RNAs from activated monomers is investigated by examining the sequence- and regioselectivity in the montmorillonite-catalyzed formation of RNA dimers and trimers. The reactivity of A was similar to that of G, and C was comparable in reactivity to U. Yet the reactivity of the purine nucleotides differed from that of the pyrimidines. In the reaction of nucleotides (pN) with activated monomers (ImpN), the sequence- and regioselectivity was Pu(3')Py > Pu(3')Pu = Pu(2')Py > Pu(2')Pu. The 5'-pyrimidine initiated dimers formed less efficiently than the 5'-purine initiated dimers. Trimer formation was investigated by the synthesis of 8 dimers (pNpN) and measuring the yields of trimers formed in the reaction of each dimer with a mixture of equal molar amounts of four activated monomers. The reactivity of the dimers depended on the nucleotide attached to the 3'-end of the RNA and the regiochemistry of the phosphodiester bond. Rules based on these studies are proposed to predict the sequence- and regioselectivity of the RNAs formed in montmorillonite-catalyzed reactions. These rules are consistent with the structures of the 2-5-mers formed in the reaction of equimolar amounts of ImpA and ImpC. This research establishes that the montmorillonite catalyst limits the number of RNA oligomer isomers formed. The potential significance of these findings to the origins of life is discussed.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region></list><tree><noCountry><name sortKey="Ferris, James P" sort="Ferris, James P" uniqKey="Ferris J" first="James P" last="Ferris">James P. Ferris</name></noCountry><country name="États-Unis"><region name="État de New York"><name sortKey="Miyakawa, Shin" sort="Miyakawa, Shin" uniqKey="Miyakawa S" first="Shin" last="Miyakawa">Shin Miyakawa</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003198 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003198 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:12837090
|texte= Sequence- and regioselectivity in the montmorillonite-catalyzed synthesis of RNA.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:12837090" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |